Red Sea Reef Colors Pro Multi Test Kit

Great value on three advanced test kits for accurate measurement of reef supplements. Red Sea Reef Colors Pro Multi Test Kit includes Red Sea Iodine Pro, Potassium Pro and Iron Pro Tests at an outstanding package price. This precision test kit boasts next generation features for greater testing convenience and accuracy perfect for new and experienced reef hobbyists.

Red Sea Reef Colors Pro Multi Test Kit contains three tests selected specifically to accurately measure chemical elements associated with coral pigmentation. Proper supplementation with iodine, potassium or iron can enhance coral coloration. However, no supplementation regimen should be employed without accurate testing and monitoring of supplement levels. Reef Colors Pro Multi Test Kit updates the testing process for ease-of-use and a high degree of accuracy that encourages proper testing skills.

Included quick guide card walks you through the testing process with simple, easy-to-understand illustrations. Routine testing with Red Sea Reef Colors Pro Multi Test Kit is crucial to the health and success of any coral reef aquarium housing SPS, LPS and Soft Corals, especially those on a supplementation regimen.

Red Sea Reef Colors Pro Multi Test Kit Contents:

• Titrator
• Instruction Manual
• 3 Color Card
• Syringe 5ml
• Syringe 1ml
• Iodine Reagent A - 25ml
• Iodine Reagent B - 50ml
• Iodine Standard - 12g
• Iron Reagent A - 12ml
• Iron Reagent B - 15g
• Potassium Reagent A - 15ml
• Potassium Reagent B - 25ml
• Potassium Reagent C - 15ml
• Potassium Reagent D - 15ml
• Filtration Kit

Why Test Iron?
Iron has a fundamental role in many biochemical metabolic processes including respiration and production of energy, chlorophyll and photosynthetic catalysts. Iron precipitates into the coral skeleton, which act as a reservoir for their use in the soft tissue. Iron is related to the green/yellow chromo-proteins (GFP) and proper iron supplementation encourages enhanced coral coloration. However, iron is toxic to all invertebrates above recommended levels and can encourage nuisance algae growth.

Why Test Iodine?
Iodine is important to the coral skeleton and soft tissue and is specifically important for SPS corals to display their natural pigments. Iodine is related to the pink chromo-protein (pocciloporin) and proper iodine supplementation encourages enhanced coral coloration. However, iodine becomes toxic in concentrations above the levels found in natural seawater.

Why Test Potassium?
Potassium has an essential role in the transportation of coral nutrients within the soft tissues including the nutrients provided by the zooxanthallae. Potassium also has a significant effect on the alkalinity inside the coral soft tissue and plays a role in the formation of aragonite in the coral skeleton. Potassium is related to the red chromo-proteins (peridinin and neo-peridinin). Proper potassium supplementation encourages enhanced coral coloration. However, overdose of potassium can encourage the breakout of nuisance algae.

Please click on "More Information" for directions for use.

1. Rinse the vial marked "standard" and syringe with RO or distilled water and dry thoroughly.
2. Using the syringe provided, place exactly 5 ml of RO water into the standard vial, close the vial firmly with the cap and place the vial into the aquarium for 10 minutes to allow the water in the vial to reach the temperature of the aquarium water.
3. After 10 min., take the standard vial out of the aquarium and add 1 level measuring spoon of Iodine standard 0.06 ppm powder. Close the vial with the cap and shake until the powder is fully dissolved.
4. Using the syringe provided, place exactly 5 ml of the water to be tested into the other vial (sample vial).
5. Add 5 drops of Iodine Pro Reagent A to both the sample and standard vials.
6. Add 8 drops of Iodine Pro Reagent B to both the sample and standard vials.
7. Stand both of the vials in their designated positions on the color card.
8. The standard vial acts as a timer for reaction. Check the color of the water in the standard vial by looking into the vial from above and watch to see when the color in the standard vial matches the color of 0.06 ppm on the color card. This is the end point of the test reaction, which will take approximately 5 - 15 minutes for an aquarium at 25°C (77°F). The warmer the water, the quicker the reaction.
9. When the end point of the test reaction has been reached, look into the sample vial from above and compare the color in the vial to the colors on the card and choose the Iodine level that gives the closest color match. If necessary, estimate an intermediate value.
10. The color in the sample vial will remain stable for 5 minutes. Do not relate to the color in the sample vial after this time.

Directions for Potassium Pro Test Kit

1. Prepare the Filtration Kit as follows:
   • Lay a clean dry Filter Paper above the Funnel
   • Place the Top Cylinder on the Filter Paper and push down, trapping the Filter Paper between the Cylinder and the Funnel
   • Place the assembled Filter onto the Filter Cup
2. Using the large syringe provided, place exactly 2 ml of the water to be tested into one of the glass vials.
3. Add exactly 3ml of RO or distilled water to the 2 ml water sample.
4. Add 4 drops of Potassium Pro Reagent A. Close the vial with the cap and shake for 15 seconds.
5. Add 11 drops of Potassium Pro Reagent B. Close the vial with the cap and shake for 15 seconds.
6. Wait for 10 minutes (use a stopwatch) and then pour all of liquid from the glass vial into the top of the filter.
7. Wait (approx 7 minutes) until at least 3ml of clear liquid has collected in the Filter Cup (above the line), remove the filter and dispose of any remaining liquid. Using the 5ml syringe, take exactly 3ml of the filtered clear liquid from the filter cup and put it into the 2nd glass vial.
8. Ensure that the dispensing tip is on the 1 ml titration syringe and fill with 0.5 ml of Potassium Pro Titrant D. Note for filling the 1ml titration syringe: Raise the plunger of the syringe until the bottom ring of the plunger is at the 1.0 ml mark. The surface of the liquid will be approximately 0.1 - 0.15 ml below the plunger. Do not try to remove the air trapped between the liquid and the plunger. This small volume of air corresponds to the liquid held inside the plastic tip.
9. Insert the syringe into the center of the Titrator so that the volumetric scale on the side of the syringe is visible. The graduations of the volumetric scale are 0.01 ml, equivalent to 3 ppm of potassium.
10. Add 2 drops of Potassium Pro Reagent C and mix gently. The test sample will now have the titration start color as shown on the instruction card.
11. Screw the glass vial onto the bottom of the Titrator.
12. Add one drop at a time of Potassium Pro Titrant D by pressing the plunger of the syringe and gently swirling after each drop, until the end color is achieved. Pay careful attention to the color of the test sample from the addition of the first drop. Note: The amount of titrant used is inversely proportional to the level of potassium in the aquarium water sample. If you reach the end color on adding the first drop, the potassium level is 467 ppm or above.
13. Note the amount of titrant used (according to the initial and final position of the plunger, not the liquid surface) and use the table to calculate the level of potassium in the water sample.
14. Dispose of any unused quantity of titrant. Rinse all syringes, glass vials and filter kit with RO or distilled water before storing. Filter Papers are for single use only and cannot be reused.

Directions for Iron Pro Test Kit

1. Using the syringe provided, place exactly 17 ml of the water to be tested into the glass vial.
2. Add a leveled measuring spoon of Iron Pro Reagent A, close the vial with the cap and shake for 15 seconds.
3. Add 6 drops of Iron Pro Reagent B. Close the vial with the cap and shake for 15 seconds.
4. Wait 15 minutes for the color in the vial to stabilize.
5. Move the vial between the designated positions on the color card, look into the vial from above and compare the color in the vial to the colors on the card. Choose the Iron level that gives the closest color match and if necessary estimate an intermediate value.
6. The color in the reaction vial will remain stable for 5 minutes. Do not relate to the color in the reaction vial after this time.

General Information

Important notes for using the Reef Colors Pro Test Kit

• Before carrying out any water testing, always check the salinity and make adjustments as necessary. If you have made adjustments to the water, wait 10 minutes for the water parameters to stabilize (e.g. 1ppt increase in salinity due to evaporation of fresh water will result in an approximate increase of 13ppm Ca).
• Before testing, clean the glass vial and the large syringe by rinsing it with the water to be tested.
• After testing, rinse all syringes and vials with RO or distilled water before storing. If vials are left unwashed, a residue can form that will affect the results of future tests. Use a slightly acidic solution such as vinegar to remove the residue.
• Close all reagents tightly immediately after use.
• The test reagents are stable up to the date stated on the pack when stored closed between 15 - 25°C (59 - 77°F).
• Store the reagents and color card in the plastic box to prevent damage from prolonged exposure to light.